Abstract
BackgroundExtensive bioactivities of alkaloids from the genus Macleaya (Macleaya cordata (Willd.) R. Br. and Macleaya microcarpa (Maxim.) Fedde) have been widely reported, as well as more and more concerned from the scientific communities. However, systematic research on the phytochemical information of M. microcarpa is incomplete. The aim of this study was to rapidly and conveniently qualitative analyze alkaloids from M. microcarpa by ultra-performance liquid chromatography/quadrupole-time-of-fight mass spectrometry (UHPLC–Q-TOF-MS) using accurate mass weight and characteristic fragment ions, furthermore separate and identify the main alkaloids, test antitumor activity in vitro and antiangiogenic activity in vivo.ResultsA total of 14 alkaloids from fruits of M. microcarpa were identified by UHPLC–Q-TOF-MS, including 5 protopines, 2 benzophenanthridines, 1 dimer, 1 dihydrobenzophenanthridines and 5 unknown structure compounds. Two major alkaloids were isolated by various column chromatographic methods. Their structures were determined by NMR data and related literatures. The two major alkaloids were evaluated for intro cytotoxic activities against HL-60, MCF-7, A-549, and in vivo antiangiogenic activity using transgenic zebrafish.ConclusionsCurrent qualitative method based on UHPLC–Q-TOF-MS technique provided a scientific basis for isolation, structural identification, and in vitro or in vivo pharmacological further study of alkaloids from M. microcarpa in the future.
Highlights
The genus Macleaya contains two species, Macleaya cordata (Willd.) R
UHPLC–quadrupole time of flight (Q-TOF)-MS is widely used in qualitative compositions
These compositions are summarized along with their retention time, theoretical mass, molecular formula, observed mass, error and MS/MS fragments combined with literature reports [15, 16] and previously isolated alkaloids from M. cordata [7, 9, 17, 18]
Summary
The genus Macleaya contains two species, Macleaya cordata (Willd.) R. Br. and Macleaya microcarpa (Maxim.) Fedde, which mainly distributed in Japan, South and Northwest China, on slopes of grass or thickets at altitudes of 450–1600 m [1]. This study aimed to rapidly and conveniently characterize alkaloids from M. microcarpa by UHPLC– Q-TOF-MS using accurate mass weight and characteristic fragment ions, separate and identify the main alkaloids by chromatographic and spectral techniques, test antitumor activity in vitro using the trypan blue method and MTT method reported previously [7], and antiangiogenic activity in vivo using transgenic zebrafish [8]. The aim of this study was to rapidly and conveniently qualitative analyze alkaloids from M. microcarpa by ultra-performance liquid chromatography/quadrupole-time-of-fight mass spectrometry (UHPLC–Q-TOF-MS) using accurate mass weight and characteristic fragment ions, separate and identify the main alkaloids, test antitumor activity in vitro and antiangiogenic activity in vivo
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