Abstract
SUMMARY 20α-Hydroxy-5β-pregnan-3-one was isolated from the extracts of enzyme or acid-hydrolysed pregnancy urine using various chromatographic procedures including gas chromatography Identification of the steroid was based upon: (1) behaviour of the free and acetylated compound, and of the products, following stereospecific enzymatic oxidations, during gas chromatography on different stationary phases, and (2) infrared spectrometry of the compound collected after gas chromatography. The gas-chromatographic behaviour of the isomers of 3-hydroxypregnan-20-one, 20-hydroxypregnan3-one and pregnane-3,20-diol and their acetates is reported.
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