Abstract
Vibrio sp. M-812 strain, isolated from marine sediment, produced an extracellular acidic polysaccharide when grown on sea water media containing sucrose. The crude polysaccharide preparation contains residues of rhamnose, galactose, glucose and uronic acid, and protein moiety.The crude polysaccharide preparation was separated into two fractions, a high molecular weight and a low molecular weight polymer, by chromatography on Sepharose 4B. A high molecular weight polysaccharide preparation was rechromatographed on Sepharose 2B and gave two peaks. These polymers fractionated by gel chromatography were heterogeneous in electro-phoresis on cellulose acetate strips.
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