Abstract
The transcription factor VaCBF2, which interacts with C-repeat/DRE and its promoter, was isolated from Vitis amurensis. The VaCBF2 amino acid sequence contained a conserved AP2 domain of 56 amino acids and a potential nuclear localization sequence. The sequence of VaCBF2 showed a high level of homology with other CBF2 family members. Phylogenetic analysis showed that the amino acid sequences may be CBF2 proteins with evolutionary relationship. Quantitative reverse-transcription polymerase chain reaction analysis indicated that the expression of VaCBF2 gene in tissues (roots, stems, leaves, and petioles) was induced by low temperature, high salinity, and application of abscisic acid and salicylic acid in a time-dependent manner but to different extents in the cold-hardy V. amurensis and the less cold-hardy Vitis vinifera. The presence of cis-elements such as MYC and ABRE in VaCBF2 promoter further confirmed that this promoter was a component of the CBF transduction pathway involved in plant response to multiple stresses.
Highlights
Abiotic stresses such as low temperature, drought, and salinity can adversely affect crop growth and development
Potential recognition motifs for C-repeat element-binding factor (CBF) protein were detected, such as DSAWR, Domain III, and Domain IV (Figure 1). These results indicated that VaCBF2 from V. amurensis was a CBF2 or tholog of other plants but with unique features
Vitis CBFs have been proven to be a nuclear protein [18], and VaCBF2 was found to be mapped in CBF/dehydration responsive element binding (DREB) subfamily of the CBF2 group through phylogenetic analysis (Figure 2)
Summary
Abiotic stresses such as low temperature, drought, and salinity can adversely affect crop growth and development. All CBF proteins bind to the C-repeat element (CRT)/dehydration responsive element (DRE) in the promoter region of downstream genes [3,4]. These proteins regulate their downstream gene expression and enhance the tolerance of plants to low temperature, drought, and high salinity. CBF4 from Arabidopsis is weakly induced by cold stress [8], whereas DREB2A is induced by drought and salinity [9] These transcript profiles suggest that CBFs have different expression levels in plants and that a degree of cross-talk exists between these pathways [10]. Dong et al / Agricultural Sciences 4 (2013) 466-472 and abscisic acid (ABA) and salicylic acid (SA) treatments
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