Isolation and Expression Analysis of Catalase Genes in Erianthus arundinaceus and Sugarcane

Abstract

Erianthus arundinaceus is one of the most important genus of sugarcane family with strong abiotic stress tolerance. In this paper, two novel full-length catalase (CAT) genes ( EaCAT-1b and SoCAT-1c, GenBank accession numbers KF864228 and KF864231) cDNA sequences were cloned by blasting the sugarcane EST database using sorghum CAT gene (GenBank accession number XM 002437586.1) cDNA sequence as a source probe. The length of cDNA for two genes was 1, 532 bp, containing 10 bp of 5′ untranslated region (UTR) and 43 bp of the 3′ UTR, and an open reading frame of 1479 bp encoding a polypeptide of 492 amino acids. High homology of cDNA (98.6 %) was found between EaCAT-1b and SoCAT-1c. Twenty-two single nucleotide polymorphisms were found in cDNA between EaCAT-1b and SoCAT-1c and eight amino acid mutation sites in the predicted proteins. The putative EaCAT-1b and SoCAT-1c protein sequence is highly conserved with sorghum, rice, corn and other species by homologous evolutionary analysis. SDS-PAGE analysis showed that the prokaryotic expression product was a fusion protein. Real-time qPCR analysis demonstrated that the expression profile of EaCAT-1b and SoCAT-1c genes was different under various drought stress time. It was also suggested CAT-1 has a different drought response mode in E. arundinaceus and sugarcane.