Isolation and determination of bacterial microbiota of Varroa destructor and isolation of Lysinibacillus sp. from it

Mehtap Usta

doi:10.1186/s41938-021-00482-7

Abstract

BackgroundThe importance of bees for environmental health is known. Within the scope of this importance, it is of great importance to protect the health of bees and to prevent colony extinction. In this context, it is very important to develop effective methods in combating microorganisms, parasitoids, mites and organisms that cause disease or harm in bees. Both use different methods in terms of bee health.ResultIn this study, the possibility of the bacteria isolated from Varroa destructor mite being bioinsecticide was investigated. Accordingly, six bacteria were isolated from the mite. Isolated bacteria were analyzed according to biochemical tests, molecular analysis, optimum growth pH and phylogenetic tree drawn as Pantoea dispersa (GV1), Lysinibacillus macroides (GV3), Bacillus mycoides (GV4), Lysinibacillus fusiformis (GV5), Pseodomonas lutea (GV5), Lysinibacillus varians (GV7). Lysinibacillus sp. The entomopathogenic feature of Lysinibacillus sp. ranked it as the most important species. When the insecticidal properties of bacteria were examined, they were determined as 53, 90, 62, 95, 74 and 83% for GV1, GV3, GV4, GV5, GV6 and GV7, respectively.ConclusionBased on these results and literature review, Lysinibacillus sp. species had a high potential to be used as bioinsecticide against V. destructor mite.

Highlights

The importance of bees for environmental health is known
Colony extinction events, which have recently been observed in different countries and whose cause cannot be explained, are considered as a problem that may affect the biological balance in the future
Isolation of bacteria Before bacterial isolation, V. destructor mites were surface sterilized by 70% alcohol to remove possible contamination and washed in sterile distilled water

Summary

Introduction

Within the scope of this importance, it is of great importance to protect the health of bees and to prevent colony extinction. In this context, it is very important to develop effective methods in combating microorganisms, parasitoids, mites and organisms that cause disease or harm in bees. It is very important to develop effective methods in combating microorganisms, parasitoids, mites and organisms that cause disease or harm in bees Both use different methods in terms of bee health. It is noted that similar problems can be seen in Turkey. Bacterial, parasitic and fungal diseases seen in honey bees are very important for the honey production sector. It is known that the rate of co-infection of honey bee colonies with colony extinction

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