Abstract
This unit describes protocols for Yersinia pestis to confirm plasmid profiles, construct and confirm a Deltapgm mutation, and cure the low-calcium response (Lcr) plasmid encoding a type III secretion system (TTSS). Strains lacking either the chromosomal pgm locus or the Lcr plasmid can be safely studied under BSL-2 conditions and are exempt from Select Agent regulations in the U.S.
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