Abstract
Aims: The present work was designed to isolate the chemical constituents from the leaf extracts of the plant Annona reticulata L. most commonly known as Ramphal or Bullock’s heart or Custard apple belongs to the family Annonaceae.
 Methods: The extraction of leaf powder was done using various solvents. The preliminary phytochemical tests were carried out using standard chemical tests. Phytochemical constituents were isolated using column chromatography and characterization of the compounds were carried out using IR, 1H NMR, and mass spectroscopy data.
 Results: The results from the preliminary phytochemical studies showed the presence of alkaloids, triterpenoids, carbohydrates,saponins and flavonoids. Isolation of leaf extracts revealed the presence of a steroid, alkaloid and flavonoid which were confirmed by spectral analysis to be α – amyrin caprylate, xylopine and fisetin.
 Conclusion: The study confirmed the presence of α – Amyrin caprylate, Fisetin and Xylopine as its main chemical constituents.
Highlights
Herbal medicine known as phytotherapy is the science of using herbal remedies to treat various sicknesses
This lead to an increase in the curiosity of the scientists and various pharmaceutical industries to develop new herbal medicine for various diseases with decreased side effects [2]
A country with highest number of plants with medicinal and therapeutic importance became a target for these pharmaceutical industries to export these plants for their plan development
Summary
Herbal medicine known as phytotherapy is the science of using herbal remedies to treat various sicknesses It covers all the aspects of herbal medicine, that is, plants with powerful actions to those with gentle actions. People have come to know of the side effects of synthetic drugs, leading to an increased development of natural products as treatment for ailments [1]. This lead to an increase in the curiosity of the scientists and various pharmaceutical industries to develop new herbal medicine for various diseases with decreased side effects [2]. The present work is to isolate the chemical constituents from the leaf extracts of the plant AR and characterize them by using IR,NMR and mass spectroscopical data
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