Isolation and characterization of twenty-three microsatellite loci in the noble scallop, Chlamys nobilis

Abstract

Twenty-three polymorphic microsatellite loci were developed from the noble scallop Chlamys nobilis, with an enriched partial genomic library. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these 23 markers ranges from 2 to 14 with an average of 6.304 per locus. The observed and expected heterozygosity values range from 0.033 to 0.833 (averaging 0.494) and 0.033 to 0.895 (averaging 0.666), respectively. Fourteen loci accord with Hardy-Weinberg equilibrium, and the rest nine significantly deviate from HWE after Bonferroni correction. Additionally, three pairs of loci show significant linkage disequilibrium. These polymorphic markers would be useful in the future for studies of population genetics, linkage mapping and other relevant research in C. nobilis.