Development of twenty-two polymorphic microsatellite loci in the noble scallop, Chlamys nobilis

Abstract

Twenty-two polymorphic microsatellites were isolated from the noble scallop, Chlamys nobilis with a partial genomic library enriched for tandem repeat sequences of (CA)(12) and (GA)(12). Polymorphism of these loci was assessed in a sample of 32 unrelated individuals. The average allele number was 7.23 per locus with a range of 3-19, and the observed and expected heterozygosity varied from 0.032-0.875 (averaging 0.452) and 0.315-0.939 (averaging 0.646), respectively. After Bonferroni correction (P > 0.0023), 11 of the 22 markers accorded with Hardy-Weinberg equilibrium, and the rest showed significant departure from HWE. Three pairs of loci showed significant linkage disequilibrium (P < 0.01). A poor transferability of these loci to other two cultured species, C. farreri and Patinopecten yessoensis was detected. These markers would be useful for studies of population genetics, linkage mapping and other relevant research in C. nobilis.