Abstract
Six microsatellite markers were developed for the larch budmoth Zeiraphera diniana Guenee 1845, using two enrichment protocols. The number of alleles ranged from 3 to 15 per locus and observed heterozygosities ranged from 0.09 to 0.98 for the 69 individuals genotyped. Using these markers significant genetic differentiation between one population from Poland and samples from Alpine populations in France and Switzerland (overall FST = 0.0298) was detected. However, the two Alpine samples did not differ significantly. These microsatellite markers are valuable tools for studying the population genetics of Zeiraphera diniana.
Highlights
The larch budmoth, Zeiraphera diniana Guénée 1845 (Lepidoptera: Tortricidae), is a Palaearctic species (Bovey & Grison, 1975)
Genetic studies have focused on the differentiation between the host races on larch and pine, using allozymes and AFLP’s (Emelianov et al, 1995, 2003a, b)
This paper reports two different microsatellite screening procedures done in two separate laboratories: Plant Research International, The Netherlands and Forest Zoology Research Unit, INRA, France
Summary
The larch budmoth, Zeiraphera diniana Guénée 1845 (Lepidoptera: Tortricidae), is a Palaearctic species (Bovey & Grison, 1975). We develop microsatellite markers to study population genetics in space and time, which will allow us to investigate the demographic processes observed
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