Abstract
1. 1. A prolactin-like hormone has been isolated from the pituitary of the grey mullet, Mugil cephalus (Linnaeus), by ultrafiltration and analytical chromatography on G-75 Sephadex. 2. 2. The hormone possessed biological activity in the Gillichthys xanthophore dispersion assay (Sage & Bern, 1971). 3. 3. A sensitive radioimmunoassay for mullet prolactin was developed and validated by comparison with biological activity data. Some immunological similarity between the ovine prolactin molecule and the mullet prolactin molecule was demonstrated by this radioimmunoassay. 4. 4. A molecular weight of 22,400 was indicated by the elution behavior of mullet prolactin from G-75 Sephadex.
Published Version
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