Abstract
Pyocyanin is a blue green phenazine pigment produced in large quantities by active cultures of Pseudomonas aeruginosa, with advantageous applications in medicine, agriculture and for the environment. Hence, in the present study, a potent bacterium was isolated from agricultural soil and was identified morphologically and by 16S rRNA sequencing as P. aeruginosa (isolate KU_BIO2). When the influence of nutrient supplements in both King’s A and Nutrient media as amended was investigated, an enhanced pyocyanin production of 2.56 µg ml−1 was achieved in King’s A medium amended with soya bean followed by 1.702 µg ml−1 of pyocyanin from the nutrient medium amended with sweet potato. Purified pyocyanin was characterized by UV-Vis Spectrophotometer and Fourier-Transform Infrared spectroscopy (FTIR). Furthermore, Liquid Chromatography Mass Spectrum (LCMS) and Nuclear Magnetic Resonance (NMR) confirmed its mass value at 211 and as N-CH3 protons resonating at 3.363 ppm as a singlet respectively. The isolated pyocyanin displayed remarkable dye property by inducing color change in cotton cloth from white to pink. Lastly, the antifungal activity of test pyocyanin showed inhibition of growth of rice blast fungus, Magnaporthe grisea and bacterial blight of rice, Xanthomonas oryzae at concentrations of 150 and 200 ppm, respectively. Thus, this investigation provides evidence for diverse actions of pyocyanin which are nutrient dependent and are capable of acting on a large scale, by utilizing microbes existing in agriculture wastes, and thus could be used as an alternative source in the making of natural textile dyes with strong durability and a broad spectrum of ecofriendly agrochemicals.
Highlights
Pyocyanin is a blue green phenazine pigment produced in large quantities by active cultures of Pseudomonas aeruginosa, with advantageous applications in medicine, agriculture and for the environment
The genetic sequence of the isolated strain (KU_BIO2), when blast searched in National Center for Biotechnology Information (NCBI), showed high similarity with non-pathogenic bacteria, further none of the pathogenic isolates of P. aeruginosa found in the NCBI database showed similarity with this isolate (KU_BIO2) (Fig. 1)
Pseudomonas aeruginosa is an opportunistic bacterium with commercial value and it is well known for its ability to secrete phenazine compounds[18]
Summary
Pyocyanin is a blue green phenazine pigment produced in large quantities by active cultures of Pseudomonas aeruginosa, with advantageous applications in medicine, agriculture and for the environment. The main purposes of this study are (i) to identify and isolate, the pyocyanin producing organism P.aeruginosa from agricultural waste soil (ii) to test productivity of pyocyanin under different nutrients and (iii) characterization of pyocyanin and its evaluation on both the dye and agrochemical properties.
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