Abstract
Abstract Three lectins, designated as Sg1, Sg2 and Sg3, were identified in the serum of the desert locust Schistocerca gregaria. With the use of (NH4)2SO4 fractionation, ion-exchange chromatography on DEAE-cellulose and affinity chromatography on CNBr-activated Sepharose 4B, three pools, each containing one of the putative lectins, were obtained. IEF, native PAGE and SDS/PAGE revealed that the three pools contained Sg1, Sg2 and Sg3, respectively, with pI = 6.39, 8.11 and 6.8; native PAGE Rm = 0.44, 0.32 and 0.32; SDS/ PAGE subunits approximate molecular weights = 21.5, 44.5, and 43.9 kDa, respectively. Under reducing conditions, SDS/PAGE has separated each of Sg2 and Sg3 into two bands that indicate the presence of covalent interactions between their subunits, which are not present in Sg1. The three lectins are more highly specific for rabbit RBCs than for those of other vertebrates. The HA of lectins are Ca2+-dependent, heat-labile, and are inhibited strongly by α-linked-D-galactosides, followed by L-(+)...
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