Abstract
The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.
Highlights
In 1875 the German Scientists found the presence of spiral bacteria in human stomachs
The urease test is based on the abundant production of urease by H. pylori
After 7 days of incubation at 37°C in a microaerophilic atmosphere, fine colonies, transparent gray and shiny, appear in the agar house, these cultural characteristics correspond to Helicobacter pylori
Summary
In 1875 the German Scientists found the presence of spiral bacteria in human stomachs. They abandoned the research because they did not arrive at the farm. H. pylori detection of bacterial urease activity These methods are either invasive requiring endoscopy with biopsy or non-invasive (Korwin and Lozniewski, 2000). Following infection with H. pylori, the main steps of the process of carcinogenesis are: Chronic active gastritis, gastric atrophy, intestinal metaplasia, dysplasia and gastric cancer (Kuipers, 1999; Peek and Blaser, 2002). A biopsy specimen is placed in the urea-indole broth for the presence of urease, two fragments in physiological saline for isolation of H. pylori and a fragment in 4% formalin include genetic methods, microbiological, for histological examination-pathological and cytological.
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