Isolation and Characterization of Kingella bonacorsii sp. nov., A Novel Kingella Species Detected in a Stable Periodontitis Subject.

Angéline Antezack,Clara Rolland,Bernard La Scola,Manon Boxberger,Virginie Monnet-Corti

doi:10.3390/pathogens10020240

Abstract

Members of the genus Kingella are mostly commensals of the oral cavity, but some of them are involved in invasive infections, especially in young children. This study provides new knowledge on the diversity of this genus by describing a novel species of Kingella isolated from a dental plaque sample from a 51-year-old man with a history of periodontitis. Morphological and chemotaxonomic characteristic were investigated using different growth conditions, pH and temperature. Cellular fatty acid methyl ester (FAME) analysis was performed by gas chromatography/mass spectrometry (GC/MS). Phylogenetic analysis based on 16S rRNA, orthologous average nucleotide identity (OrthoANI) and digital DNA–DNA hybridization (dDDH) relatedness were also performed. Strain Marseille-Q4569T was found to be a facultative aerobic, nonmotile and non-spore-forming rod-shaped bacterium that grows at 28–41.5 °C (optimum 37 °C), pH 5.5–8.5 (optimum pH 7.5) and 5–15 g/L of NaCl. The major fatty acids were Hexadecanoic acid (32.7%), 11-Octadecenoic acid (26.1 %) and 9-Hexadecenoic acid (21.3 %). Despite high 16S rRNA gene sequence similarity (98.72%) between strain Marseille-Q4569T and Kingella oralis strain UB-38T, the degree of OrthoANI was at the limit of the cutoff (95.83%), and the degree of dDDH was lower (63.6%) than thresholds used to delineate prokaryotic species. Therefore, it is proposed that strain Marseille-Q4569T represents a novel species of the genus Kingella, for which the name Kingella bonacorsii sp. nov. is proposed (=CSUR Q4569).

Highlights

Periodontitis is a chronic inflammatory disease characterized by microbially associated, host-mediated inflammation, which leads to the progressive destruction of the supporting structures of the teeth [1]
Periodontitis can be successfully treated through control of local and systemic risk factors, but stable periodontitis patients must be closely monitored, as they remain at higher risk for recurrent disease
We propose to establish for this strain the species name Kingella bonacorsii sp. nov

Summary

Introduction

Periodontitis is a chronic inflammatory disease characterized by microbially associated, host-mediated inflammation, which leads to the progressive destruction of the supporting structures of the teeth [1]. Clinical gingival health after successful treatment of periodontitis was defined by a bleeding on probing < 10%. Of sites, shallow probing depths of 4 mm or less and no 4 mm sites with bleeding on probing, optimal improvement of other clinical parameters and absence of progressive periodontal destruction [2]. The health-associated microbiome is predominantly dominated by Streptococcus, AcThe health-associated microbiome predominantly dominatedspecies by Streptococcus, Actitinomyces, Rothia, Lautropia, Bergeyella,isKingella and Granulicatella [3]. The genus nomyces, Rothia, Lautropia, Kingella and Granulicatella species [3].

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Conclusion