Abstract
Capnocytophaga species are commensal gliding bacteria that are found in human and animal oral microbiota and are involved in several inflammatory diseases, both in immunocompromised and immunocompetent subjects. This study contributes to increased knowledge of this genus by characterizing a novel species isolated from a dental plaque sample in a male with gingivitis. We investigated morphological and chemotaxonomic characteristics using different growth conditions, temperature, and pH. Cellular fatty acid methyl ester (FAME) analysis was employed with gas chromatography/mass spectrometry (GC/MS). Phylogenetic analysis based on 16S rRNA, orthologous average nucleotide identity (OrthoANI), and digital DNA–DNA hybridization (dDDH) relatedness were performed. The Marseille-Q4570T strain was found to be a facultative aerobic, Gram-negative, elongated, round-tipped bacterium that grew at 25–56 °C and tolerated a pH of 5.5 to 8.5 and an NaCl content ranging from 5 to 15 g/L. The most abundant fatty acid was the branched structure 13-methyl-tetradecanoic acid (76%), followed by hexadecanoic acid (6%) and 3-hydroxy-15-methyl-hexadecanoic acid (4%). A 16S rDNA-based similarity analysis showed that the Marseille-Q4570T strain was closely related to Capnocytophaga leadbetteri strain AHN8855T (97.24% sequence identity). The OrthoANI and dDDH values between these two strains were, respectively, 76.81% and 25.6%. Therefore, we conclude that the Marseille-Q4570T strain represents a novel species of the genus Capnocytophaga, for which the name Capnocytophaga bilenii sp. nov. is proposed (=CSUR Q4570).
Highlights
Capnocytophaga species are primarily commensals of the oral cavity in humans and animals, especially dogs and cats. They are recognized as opportunistic pathogens, leading to various extra-oral infections, including severe sepsis [2], bloodstream infections [3], abscess [4,5], vertebral osteomyelitis [6], pneumonia [7], and perinatal infections [8] in both immunocompetent and immunosuppressed patients
Capnocytophaga gingivalis has been identified as strongly correlated with oral squamous cell carcinoma (OSCC) and has been described as a promising diagnostic marker [9,10]
Marseille‐Q4570 was isolated from a dental plaque sample of a 25‐year‐
Summary
Capnocytophaga species are primarily commensals of the oral cavity in humans and animals, especially dogs and cats. They are recognized as opportunistic pathogens, leading to various extra-oral infections, including severe sepsis [2], bloodstream infections [3], abscess [4,5], vertebral osteomyelitis [6], pneumonia [7], and perinatal infections [8] in both immunocompetent and immunosuppressed patients. Capnocytophaga gingivalis has been identified as strongly correlated with oral squamous cell carcinoma (OSCC) and has been described as a promising diagnostic marker [9,10]. An increase in an abundance of Capnocytophaga of Capnocytophaga species was found in subjects with gingivitis [16] and periodontitis [17].
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