Abstract
Hydrocarbon utilizing yeasts (HUY) were isolated from palm wine, a rich sugar substrate from a non-oil impacted environment using Bacto Bushnell Haas broth. The medium composed of 0.2 g MgSO4, 0.02 g CaCl2, 1 g KH2PO4, 1 g K2HPO4, 0.05 g FeCl2, 1 g/L NH4NO3, pH 7.2 ± 0.2) and mineral salt broth comprising of 4.4 g KH2PO4, 8 g NH4)2SO4, 2 g Na2HPO4.H2O, MgSO4.7H2O, 0.1g FeSO4, 0.01 g/L CaCl2, pH 4.5 ± 0.2) supplemented with palm wine (0.5%) and crude oil (1%). Yeasts isolated from an enriched medium and palm wine showed similarities. Yeast isolates were further screened for hydrocarbon degrading potentials using the Bacto Bushnell Haas broth containing 2,6- dichlorophenol indophenols as the indicator agent. The isolates were identified using microscopic, macroscopic, fermentation and molecular characteristics as Candida adriatica (ZIM 2468) and Candida taoyuanica (MYA-4700).
Highlights
Diverse microbial population’s bacteria, yeasts or molds have been reported for degradation of hydrocarbons
The crude oil used in this research was the Nigerian Bonny light crude oil obtained from Shell Petroleum Development Company (SPDC) Limited, Port Harcourt, Nigeria
Results from the enrichment medium and the palm wine showed that microorganisms were of the same type based on colonial and cellular morphologies (Table 1)
Summary
Diverse microbial population’s bacteria, yeasts or molds have been reported for degradation of hydrocarbons. Yeasts are eukaryotic microorganisms, classified in the kingdom fungi, with about 1500 species currently identified and described. They are estimated to be 1% of all fungal species [2] and measures up to 3-4 μm in diameter some can reach over 40 μm depending on the species [3]. Oxidative yeast (film yeasts) may grow as a film or scum on the surface of a liquid medium where as fermentative yeasts grow throughout the liquid [4] They are chemoorganotrophs, use organic compounds as source of energy [5], they could be aerobes, facultative anaerobes but never strict or obligate anaerobes [6,7]
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