Abstract
ABSTRACTObjective: To investigate the antioxidant activity of methanolic extract of endophytic fungi isolated from Calotropis procera plant.Methods: The endophytic fungi were isolated from explants of leaf, stem, and flower of C. procera on potato dextrose broth (PDB) medium. The fungalisolates were mass cultured in PDB. The methanolic extracts of these fungi were prepared and evaluated for the antioxidant activity by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and reducing power assay. The extracts were characterized for the presence of phenolics andflavonoids.Results: A total of 8 endophytic fungi were isolated from C. procera explants. Out of these 8 fungal isolates, the crude extracts of two fungi, i.e. CPIMR-2and CPIL-1 showed positive antioxidant activity. From the morphological characteristics, the isolates CPIMR-2 and CPIL-1 were identified as Penicilliumspp. and Aspergillus spp., respectively. Both the isolates were found to possess antioxidant potential with % inhibition value of 81.16% and 80.97%in the DPPH radical scavenging assay. The phytochemical screening of the methanolic extracts showed the presence of phenolics and flavonoids. Thetotal flavonoids content in Penicillium species and Aspergillus species were found to be 130.50 μg/mg and 94.91 μg/mg of extracts, respectively. Thetotal phenolic content was found to be 9.16 μg/mg and 12.13 μg/mg of extracts in Penicillium species and Aspergillus species, respectively.Conclusions: Endophtyic fungi were found to be present in medicinal value plant C. procera. The probable bioactive component for antioxidantactivity possessed by the fungi would be the flavonoids and phenolics. These metabolite produced by endophytic fungi from C. procera need to beexplored further as potential source of novel natural antioxidant compound.Keywords: Endophytes, Calotropis procera, Antioxidant, Radical scavenging, Reducing power, Flavonoids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
