Isolation and Characterization of Cellulase Secreting Bacterium from Cattle Manure: Application to Composting

Abstract

A extracellular cellulase producing bacterium was isolated from cow feces, and was identified to be Bacillus licheniformis on the basis of morphological and biochemical properties as well as the composition of cellular fatty acids composition (FAME). CMCase, FPase and avicelase activities of the isolates cultured in CMC media at 37°C for 24 hrs were 1.65 U/ml, 0.13 U/ml and 0.18 U/ml, respectively. However, β-glucosidase activity was not detected. The optimum pH and temperature for induction of the enzyme were 7.5 and 50°C. The maximum CMCase activity was observed at pH 7.5 and 75°C. Zymogram analysis for crude supernatant showed four major bands CMC-SDS-PAGE. By adding 0.1% DMP (Developed Microbial Product) -comp including B. licheniformis NLRI X-33, microbial product, to swine manure composting pile, the gab of composting temperature within the pile in DMP-comp and control was 23°C and 34°C, respectively, during the initial 23 day composting periods. Coliforms were not found in the swine + sawdust samples after 30 days of DMP-comp treated and 40 days of control under forced aeration system.