Abstract

A hitherto unreported esterified form of steryl glucoside has been isolated from potato tuber lipids and soybean phosphatides. It is composed of sterol, glucose, and fatty acid in the molar ratio 1:1:1. Four components of the sterol moiety have been detected ; they include Β-sitosterol and stigmasterol (identified by gas–liquid chromatography). Fatty acids are palmitic, stearic, oleic, linoleic, and linolenic acids. As demonstrated by infrared analysis and periodate oxidation, the fatty acid is located on C6 of the sugar portion.

Highlights

  • 1N A PREVIOUS paper [1] the presence of free and esterified forms of steryl glucosides in potato lipid extracts was reported

  • Thin-layer chromatography showed a single component of R F 0.07 in HEA and R F 0.91 in diisobutyl ketone-acetic acid-water :50 :10 (DKAW). Aliquots of this product were treated with 0.1 N methanolic potassium hydroxide for 15 min at 37' and fatty acid methyl esters ( K F 0.67 in HEA) and a derivative of same RF as steryl glucoside were shown by thin-layer chromatography (TLC)

  • esterified steryl glucoside (ESG) is very heterogeneous within the class

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Summary

Introduction

1N A PREVIOUS paper [1] the presence of free and esterified forms of steryl glucosides in potato lipid extracts was reported. A commercial source of soybean phospholipids, containing appreciable quantities of esterified steryl glucoside (ESG), was used as starting material. Revealed by TLC, were: fatty acid methyl esters (RF 0.63 in hexane-diethyl ether-acetic acid 90: 10:1 (HEA)), and the deacylated product, which had the same RF value as steryl glucoside (0.46 in DKAW).

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