Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana).

Bing Tan,Yong Li,Wei Zhang,Li-Jun Wu,Guo-Xiang Yang,Bei Li,Zheng-Li Shi,Jing Chen,Yong-Song Lei,Guang Chen,Han-Zhong Wang,Xing-Lou Yang

doi:10.1186/s12985-016-0648-6

Bing Tan, Yong Li + Show 10 more

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https://doi.org/10.1186/s12985-016-0648-6

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Abstract

BackgroundAdenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates. Although many adenoviruses have been identified in monkeys, the knowledge of these viruses from the Colobinae members is quite limited.FindingsWe conducted a surveillance of viral infection in endangered golden snub-nosed monkeys (Rhinopithecus roxellana) in the subfamily Colobinae in China, and found that 5.1% of sampled individuals were positive for adenovirus. One of the adenoviruses (SAdV-WIV19) was successfully isolated and its full-length genome was sequenced. The full-length genome of WIV19 is 33,562 bp in size, has a G + C content of 56.2%, and encodes 35 putative genes. Sequence analysis revealed that this virus represents a novel species in the genus Mastadenovirus. Diverse cell lines, including those of human origin, were susceptible to WIV19.ConclusionWe report the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0648-6) contains supplementary material, which is available to authorized users.

Highlights

Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates
Body of text Within the family Adenoviridae, the genus Mastadenovirus contains a group of non-enveloped icosahedral viruses that range in size from 70 to 90 nm and contain a linear double-stranded DNA genome of approximate 35 kb [1]
* Correspondence: zlshi@wh.iov.cn 1Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China 3University of Chinese Academy of Sciences, Beijing, China Full list of author information is available at the end of the article have been associated with diarrhea, acute respiratory illness, pneumonia, and hepatitis in captive non-human primates [4,5,6]

Summary

Introduction

Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates. * Correspondence: zlshi@wh.iov.cn 1Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China 3University of Chinese Academy of Sciences, Beijing, China Full list of author information is available at the end of the article have been associated with diarrhea, acute respiratory illness, pneumonia, and hepatitis in captive non-human primates [4,5,6]. Golden snub-nosed monkeys (Rhinopithecus roxellana) living in Shennongjia Nature Reserve (SNR) in Hubei, China, are an endangered species belonging to the subfamily Colobinae [14].

Results

Conclusion