Abstract
BackgroundBreast cancer is one of the most common cancer and a leading cause of death in women. Up to date the most commonly used breast cancer cell lines are originating from Caucasians or Afro-Americans but rarely cells are being derived from other ethnic groups. Here we describe for the first time the establishment of a naturally transformed breast cancer cell line, KAIMRC1 from an Arab woman of age 62 suffering from stage IIB breast cancer (T2N1M0). Moreover, we have characterized these cells for the biological and molecular markers, induction of MAPK pathways as well as its response to different commercially available drugs and compounds.MethodsBreast cancer tissue sections were minced and cultured in media for several weeks. KAIMRC1 cells were successfully isolated from one of the primary breast tumor tissue cultures without any enzymatic digestion. To study the growth characteristics of the cells, wound healing assay, clonogenic assay, cell proliferation assays and live cell time-lapse microscopy was performed. Karyotyping, Immunophenotyping and molecular pathway specific compound treatment was also performed. A selective breast cancer gene expression panel was used to identify genes involved in the signal transduction dysregulation and malfunction of normal biological processes during breast carcinogenesis.ResultsThese cells are ER/PR-positive and HER2-negative. The epithelial nature of these cells was confirmed by flow cytometry analysis using epithelial cell markers. They are cuboidal in shape and relatively smaller in size as compared to established cell lines, MCF-7, MDA MB-231 and the normal breast cell line, MCF-10A. In normal cell culture conditions these cells showed the capability of growing both in monolayer as well as in 3-D conformation. They showed a doubling time in vitro of approximately 24 h. They exhibit a modal karyotype of 58–63,X with abnormalities in a couple of chromosomes. KAIMRC1 cells were found to be more responsive to drug treatment in vitro in comparison to the established MDA MB-231 and MCF-7 cell lines.ConclusionsIn conclusion we have isolated and characterized a new naturally immortalized breast cell line, KAIMRC1 with a potential to play a key role in opening up novel avenues towards the understanding of breast carcinoma.
Highlights
Breast cancer is one of the most common cancer and a leading cause of death in women
Characterization of KAIMRC1 cells Breast cancer tissue sections were obtained following consent from several patients diagnosed with breast carcinoma
They displayed cuboidal-like morphology (Fig. 1a), made dome like colonies in-vitro (Fig. 1b), and were relatively smaller in size compared to the established cell lines, Michigan Cancer Foundation-7 (MCF-7), MDA MB-231 and the normal breast cell line, MCF-10A (Fig. 2s-v)
Summary
Breast cancer is one of the most common cancer and a leading cause of death in women. Different experimental models are being used to study breast cancer that includes animal models, cell lines and biopsies from the tumor itself. Gazdar and colleagues, developed a panel of tumor cell lines along with paired non-malignant cell lines or strains collected from breast cancers, predominantly primary tumors [8] This included 18 cell lines derived from primary tumors and three derived from metastatic lesions and for the majority of them they established one or more corresponding non-malignant cell strains. Another study, [9] described the development of five breast cancer cell lines from a breast cancer tissue derived from a single patient These were derived from a single tumor, all five breast cancer cell lines displayed different antigenic expression profiles, tumorigenicity and organ specific metastatic abilities
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call