Abstract
The ability to accumulate S-adenosylmethionine (SAM) of 572 yeast strains isolated from environmental sources were surveyed. An S-adenosylmethionine enriching strain S42-12, identified asCandida sp., was chose to develop a SAM-accumulating mutant successfully. The final SAM-accumulating mutant strain YQ-5 was isolated by UV radiation or by NTG treatment using ethionine selection and nystatin selection method. The mutant strain YQ-5 accumulated 112.1 mg per gram biomass, was 3.14-fold higher than the original strain S42-12. When cultivated in the optimal medium with a favourable fermentation conditions, SAM content of the mutant strain reached at 1740 mg L−1. Trend of SAM and ergosterol contents and methionine adenosyltransferase activity of SAM-accumulating mutants during fermentation were analysed. The results suggested that one of the reasons why the mutants accumulated SAM in significantly high amounts may be the lower consumption of SAM for ergosterol biosynthesis, other than improvement of methionine adenosyltransferase activity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
