Abstract
A rat genomic DNA (SOD1) encoding Cu/Zn Superoxide dismutase (SOD1) (superoxide; Superoxide oxidoreductase, EC 1.15.1.1) was cloned and sequenced. The rat SOD1 gene consisted of five exons and four introns spanning about 6 kb. The transcription start point ( tsp) was observed 93 bp upstream from the ATG codon by primer extension analysis. The 5′-flanking sequence of SOD1 contained two CCAAT box motifs, a TATA box and four GC-like boxes. In the 3′-flanking region of SOD1, a polyadenylation signal, consensus sequence YGTGTTYY, and a G/T cluster were observed. A rat identifer ( ID) sequence, a repetitive element of the rat genome, was located at between 569 and 484 bp upstream from the tsp.
Published Version
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