Isolated culture of A. reptance L., its’ morphological and growth features

Elen Poghosyan,Margarit Petrosyan,Karen Trchounian,Naira Sahakyan,Irina Batlutskaya

doi:10.1051/bioconf/20214001001

Elen Poghosyan, Margarit Petrosyan + Show 3 more

Open Access

https://doi.org/10.1051/bioconf/20214001001

Copy DOI

Abstract

A growing demand for the ecologically pure products brings us for searching novel biotechnological approaches for plant cultivation. One of these approaches is the in vitro cultivation and further acclimatization of valuable plant species. The object of our investigation was Ajugareptance L. ornamental plant which possesses high metabolic activity. In vitro cultivation was carried out applying Murashige-Skoog nutrient medium and its modifications. Acclimatization of in vitro plants was implemented according Hazarika. In the presence of twice higher concentration of cytokinins over auxins and 0.2 mg/ml gibberellins callus culture was formed from the leaf explants. Callus tissue was formed in the presence of 0.2 mg/ml kinetin and 2 mg/ml indole-3-acetic acid which has denser structure than the first one. The shoot formation was observed on callus cultures growing on the same medium approximately after 5th passage. Callus culture growth was supported also by the adding of 2 mg/ml 2,4-dichlorophenoxyacetic acid. For the micropropagation, the already formed shoots were transferred to the nutrient medium which contains only 0.1 mg/ml 1-Naphthaleneacetic acid as a phytohormone. A. reptans culture has high regenerative ability and the micro-propagation index was 104 – 105. In vitro regenerated plants were successfully acclimatized to the soil conditions during two-week period.

Highlights

A growing demand for the ecologically pure products brings us for searching novel biotechnological approaches for plant cultivation
Previous studies of the same author have shown that the production of exosteroids in A. reptans culture depends on the level of culture differentiation and calluses derived either from the leaves or roots are not able to synthesize exdosteroids[22, 23].In other reports it is mentioned that A. reptans isolated culture can serve a source of ecdysteroids but there occur a tendency to decrease the synthesis of these substances in parallel to increase of cultivation period[20]
Callus culture formed on A. reptance leaf explants both on MS and N7 nutrient media

Summary

Introduction

A growing demand for the ecologically pure products brings us for searching novel biotechnological approaches for plant cultivation. Plants belonging to the Ajuga genus,in addition to their ornamental role, possess high metabolic activity [8 -10].Some species of this genus are categorized as endangered [10]. Many species of this genus produce substances with cytotoxic, insecticidal, antibacterial, antiviral, antioxidant agents [10 -12]. Many researchers state that A. reptance is characterized by the presence of 7 basic ecdysteroids (ayugalactone, cyasterone, sengosterone, 29-norsengosterone, 29norcyasterone, 20E and polypodine B) [18,19,20] These substances are produced in different quantities depending on the cultivation conditions. Previous studies of the same author have shown that the production of exosteroids in A. reptans culture depends on the level of culture differentiation and calluses derived either from the leaves or roots are not able to synthesize exdosteroids[22, 23].In other reports it is mentioned that A. reptans isolated culture can serve a source of ecdysteroids but there occur a tendency to decrease the synthesis of these substances in parallel to increase of cultivation period[20]

Methods

Results

Conclusion