Isolasi dan Uji Aktivitas Antioksidan Minyak Atsiri Daging Buah Pala (Myristica fragrans)

Abstract

Nutmeg is considered an important ingredient of various industrial applications ranging from food to cosmetics. The pharmaceutical product is also important for its antioxidant and antimicrobial properties. Further research is needed to isolate and identify the bioactive compounds of Myristica fragrans. The aim of this research was to isolate and determine the antioxidant activity of the essential oil of nutmeg flesh (Myristica fragrans). The sample used was nutmeg flesh (Myristica fragrans) taken from Ternate Island, North Maluku. The essential oil of nutmeg flesh was isolated using water vapor distillation and the antioxidant activity was tested using the DPPH method. All data are presented as mean ± standard deviation (SD) and were carried out in triplicate. The results of the research showed that the isolation of nutmeg essential oil obtained with a yield of 0,32%. Organoleptically, the essential oil of nutmeg flesh obtained from the steam distillation process has quality that meets SNI, in the form of a clear to rusty liquid and has a distinctive odor. Phytochemical tests obtained that the essential oil of nutmeg flesh contains alkaloids, flavonoids and steroids, and does not contain polyphenols and saponins. The concentration of essential oil increases, the absorption value and percent inhibition increase so that an average IC50 value of 49,34 ppm is obtained. The essential oil of nutmeg flesh by steam distillation obtained a yield of 0,32% and has antioxidant activity which is classified as a strong antioxidant