Isoelectric focusing of proteins and some enzymes from secondary phloem of cherry grafting-combinations. III. Enzymes, especially peroxidases

Abstract

Peroxidases, esterases and polyphenoloxidases in secondary phloem of grafting unions of P. avium/P. avium, P. avium/P. fruticosa and P. avium/P. cerasus combinations were determined by isoelectric-focusing in winter and spring. Polyphenoloxidases could not be detected. Some small esterase bands could be seen in winter phloem after isoelectric focusing and developing, but these results did not allow prediction about specific relations of the Prunus species examined or of their combinations. The total number of peroxidases, as well as their concentrations, were found to be generally lower in spring than in winter. Most isoperoxidases in the anodic region present during dormancy disappeared and new ones appeared in this region after break of dormancy. In contrast to the P. avium/P. avium combination, the rootstocks of the P. avium/P. fruticosa and the P. avium/P. cerasus graftings in winter showed well differentiated band patterns in the cathodic region. These patterns could also be seen in some scions of these combinations, but they were smaller. There seems to be a connection between these peroxidase bands and the growing-intensity of these species.