Abstract
This chapter discusses high-resolution full-range isoelectric focusing (IEF) of proteins and peptides in capillary electrophoresis (CE). IEF is a method that separates proteins on the basis of their isoelectric points (PI). It is accomplished by the electrophoresis of proteins or peptides through a stable pH gradient until their net charge is zero. At this pH, mobility is zero, and the isoelectric point is achieved. This method has been widely used in research, pharmaceutical, and clinical areas to separate, isolate, and analyze proteins from various biological products. The chapter discusses the effects of sample buffer matrix, surfactants, denaturant, and narrow pH range ampholyte using the same CE-IEF method. The study indicated that the CE-IEF method developed by Chen and Wiktorowicz has proven to be very flexible. This CE-IEF method used on a free solution separation can be used to perform rapid high-resolution analysis of proteins and peptides with various sample buffer matrices and additives.
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