Isoelectric focusing of proteins and some enzymes from secondary phloem of cherry grafting-combinations. II. Proteins in spring

Abstract

Buffer-soluble proteins in secondary phloem surrounding the xylem in grafting-unions of P. avium/P. avium, P. avium/P. cerasus and P. avium/P. fruticosa combinations were extracted and isoelectric-focused in spring. The total number of proteins as well as their concentrations were found to be generally lower in spring than in winter. In all graft combinations, except Hed./(F 12/1) and Hed./Cer. W 11, the number of proteins in the sections adjacent to the graft union was reduced. However, Hed./(F12/1) showed a rise to a maximum in the section below the union. The proteins of rootstock sections located at a greater distance from the union were relatively high in 3 combinations (Hed. on Frut., Cer. W 10 and F 12/1) as compared to those of the scion, whereas the other combinations did not show marked differences in this respect.