Abstract
Specific, high affinity binding sites for iodinated endothelin-1 ([ 125I]-ET-1) were identified in crude plasma and light membrane fractions harvested from aerobically perfused and ischaemic rat hearts, to determine whether the ischaemia-induced increase in binding site density (B max) involves externalization of the sites. In crude plasma membranes B max increased after 60 min ischaemia, from 113.5 ± 2.15 to 180.6 ± 4.67 fmol/mg protein (p < 0.01). In the light membranes, the B max fell, from 94.7 ± 8.70 to 63.80 ± 6.26 fmol/mg protein (p < 0.05). Hill coefficients and selectivity of both membrane fractions were unchanged. These results are interpreted as meaning that ischaemia causes externalization of cardiac [ 125I]-ET-1 binding sites.
Published Version
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