Is there a relation between stromal-derived factor 1 polymorphism and ITP chronicity in Egyptian children?

Abstract

Background Primary immune thrombocytopenia (ITP) is the most frequent hemorrhagic disease of childhood. It is an autoimmune disease characterized by abnormally increased destruction of platelets and decreased production of megakaryocytes. Stromal-derived factor-1 (SDF-1) plays a role in megakaryopoiesis and may be involved in the pathogenesis of ITP. Aim The aim of this study was to find out the prevalence of single-nucleotide polymorphism (SNP) of SDF-1 gene rs2297630 A&z.urule;G in children with ITP and its relation to chronicity. Participants and methods SNP of the SDF-1 gene (rs2297630) was assessed in 100 ITP children: 50 chronic and 50 nonchronic ITP children (acute and persistent) attending Alexandria University Children’s Hospital in Egypt and in 50 healthy controls of matched age and sex. The genotype was analyzed by PCR after DNA extraction from peripheral blood samples and confirmed by direct sequencing. Results In this study, analysis of the relationship between SDF-1 polymorphisms and clinical features showed that Rs2297630 A/G is not associated with protection from chronicity or steroid dependence in the studied patients [(χ 2=0.0, P=1.0) and (P=0.21)]. respectively Conclusions ITP is not uncommon in Egyptian children and has a great financial and psychosocial burden on our community. AG genotype of the SNP (rs2297630) of the SDF gene has no significant relation to ITP chronicity or steroid dependence in the studied groups.