Abstract
Opinion
Highlights
Use of Microsomes and Hepatocytes in Drug Discovery and Development subcellular fractions for drug metabolism studies as it is inexpensive and easy to handle [4]
In order to determine intrinsic clearance of new chemical entities (NCEs), which is a useful parameter for screening process of stable compounds and for establishing an in vitro correlation between animals and humans, microsomal assays play significant role
There are few limitations of microsomes, for instance, they have to be supplied with co-factors like NADPH, or UDPGA for the metabolic reactions to initiate, and lack the cell membranes to mimic the physiological environment in hepatocytes [7, 8]
Summary
Use of Microsomes and Hepatocytes in Drug Discovery and Development subcellular fractions for drug metabolism studies as it is inexpensive and easy to handle [4]. Microsomal assays are the default assays for metabolism and DDI studies at the drug discovery stage [5]. This assay can be utilized to assess extra-hepatic metabolism and determine the DDI potential of the drug candidate in preclinical phase [6].
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