Is the plasma membrane of the human oocyte reorganised following fertilisation and early cleavage?

Abstract

The purpose of the present study was to determine whether the plasma membrane of the human oocyte is reorganised following fertilisation and during early cleavage. In order to characterise and localise the major sugar moieties on surface glycoproteins, oocytes and embryos were labelled with a range of fluorescent lectins. Regional organisation of plasma membrane microvilli in oocytes and embryos was also studied using scanning electron microscopy (SEM). The plasma membrane of human oocytes, zygotes and early blastomeres stained strongly and homogeneously with concanavalin A and Triticum vulgaris lectin (WGA), indicating the presence of plasma membrane glycoconjugates with alpha-D-mannosyl residues, sialic acid and beta-NAc-glucosaminyl groups. We did not observe regional domains in oocytes and zygotes, suggesting that the plasma membrane is not topographically reorganised following fertilisation. SEM shows the surface of the human zygote to be organised into short microvilli 0.2-3.0 microns in length and at a density of 5-20/microns2. In early cleavage stages the microvilli are shorter and less frequent (0.2-1.0 microns; 1-5/microns2); however, there is no evidence of polarisation at this level of organisation, at either stage of development. The surface of cell fragments, common in the human embryo in vitro, differs in having few microvilli and numerous cytoplasmic blebs. In conclusion, there are no obvious morphological signs of regionalisation in the plasma membrane of the human embryo before the 8-cell stage.