Abstract
Rhizobium SBS-R100, isolated from the stem nodules ofSesbania procumbens, synthesized β-galactosidase constitutively. Transposon mutagenesis by Tn9 induced mutants defective in lactose utilization; the mutations did not interfere with growth, nodulation or N2 fixation. Mouse monoclonal antibody raised against β-galactosidase ofEscherichia coli reacted with soluble proteins of wild typeRhizobium SBS-R100. Anin vivo constructed recombinant plasmid pSBS-4 complemented aRhizobium mutant defective in lactose utilization.
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