Abstract
Sporothrix chilensis is a mild-pathogenical specie of Sporothrix pallida complex, until now, known as restrict to Chile. Herein, we describe the first clinical isolates identified as S. chilensis in Brazil, preserved in the URM Culture Collection, by polyphasic taxonomy, and their respective antifungal profile of this emergent fungus.
Highlights
Isolation and molecular screening of isolates preserved in fungal collection All isolates were recovered from the URM Culture Collection and pure cultures with macroscopically characteristic of Sporothrix species were found in Sabouraud Dextrose Agar (SDA) slants at 30 ̊C
Microscopic observation at this time revealed the presence of simple, ovate hyaline and thin-walled conidia in sympodial conidiophores, and a second brown, thick-walled dark conidia along the undifferentiated hyphae
In this molecular screening seven isolates were identified as S. schenckii sensu stricto and four isolates were confirmed to be members of the S. pallida complex (Fig 1)
Summary
Sporotrichosis is a subcutaneous fungal infection with worldwide distribution, presenting some endemic areas in tropical and subtropical regions, occurring preferably Latin America. JCNE E-26/203.301/2017), by Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico [CNPq] AMD0070-2.00/2016) and Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico [CNPq] The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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