Abstract
Background:Radiation therapy plays an important role in the treatment of lung cancer.Howeverit also radiation therapy often induces serious complications such as radiation-induced pneumonitis and its underlying mechanism remains to be elucidated. Objectives:The present study examined whether radiation might stimulate human lung fibroblasts(HLFto release neutrophil and monocyte chemotactic activity(NCA and MCA. Methods:HLF were exposed to varying doses of radiation (3-12 Gyat varying incubation times(12-72 hr. The chemotaxis assays of NCA and MCA were performed by a 48-well microchemotaxis chamber method. The effect of polyclonal antibodies of various cytokines on NCA and MCA was evaluated.Furthermorethe protein and mRNA expression of these effective cytokines was assessed by ELISA and RT-PCR. Results:HLF released NCA and MCA in response to irradiation in a dose-and time-dependent manner.NCA was significantly inhibited by anti-interleukin (IL-8 antibody and MCA was significantly attenuated by anti- monocyte chemoattractant protein (MCP-1 antibody. The protein secretion of IL-8 and MCP-1 was significantly increased by irradiationand mRNA expression of IL-8 and MCP-1 was upregulated by irradia‑ tion. Conclusion:These findings suggest that HLF mayat least partlyparticipate in the development of radiation- induced pneumonitis.Shinshu Med J 58 : 57―68 2010 (Received for publication November 262009 ;accepted in revised form January 82010
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