Abstract
Considering on required detection time and sensitivity, electrochemical method is an excellent candidate for ascorbic acid (AA) sensing. We propose using the synergistic effects of iron(0)/iron(III) oxide decorated on the electrochemically reduced graphene oxide (ERGO/Fe-Fe2O3) modified Pt microelectrode as an electrochemical AA sensor using cyclic voltammetry (CV) technique. Herein, ERGO/Fe-Fe2O3 was directly fabricated on the Pt microelectrode using a novel one-step electrosynthesis. Fe-Fe2O3 acts as an oxidized-nanozyme with works as redox centers on the electrode for oxidation of AA. Fe-Fe2O3 nanozymes are immobilized on electrochemically reduced graphene oxide (ERGO), which supports a large electroactive and excellent electrically conductive surface for electron-transfer during electrochemical oxidation of AA. The developed electrochemical sensor allowed for sensing AA in medical samples with high sensitivity in concentration range from 0.05 to 10.00 mM and a limit of detection (LOD) of 5.93 μM.
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