Abstract
This study covers the effect of ferrous chloride (FeCl2) concentration on the formation of iron oxide nanoparticles (IONPs) via the precipitation method. IONPs with appropriate surface functionalization were synthesized in order to obtain a stable colloidal IONPs (ferrofluid) for the conjugation process. The electrostatic repulsion using percloric acid (HClO4) and steric stabilization using silane polyethelene glycol (SiPEG) were generated. The optimum concentration of FeCl2 was at 0.3 M. From the transmission electron microscopy (TEM) image, the size of IONPs obtained was ~13 nm. The stable IONPs were then conjugated to the antibody and tested in the lateral flow immunoassay as the labelling agent. The conjugated IONPS with the antibody was proven sensitive to the Brugian Filiarisis disease.
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