Abstract
In Escherichia coli some of the responses induced by DNA-damaging agents involve a set of functions known as the SOS responses as well as damage-inducible genes. The SOS responses can be detected with the SOS chromotest in which the tester strain E. coli PQ37 is used. This strain carries a sfiA::lacZ fusion lacing lacZ, the structural gene for the beta-galactosidase, under control of the sfiA gene which is one of the damage-inducible genes, and has a deletion for normal lac region so that beta-galactosidase activity is strictly dependent on sfiA expression and can be taken as an indicator of DNA-damaging agents.
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