Abstract
Recent studies showed that elevated free fatty acids (FFAs) can induce endoplasmic reticulum (ER) stress, and this, in turn, plays a role in the development of insulin resistance (IR). This work aimed to verify the effects of palmitate on GLUT4 and ER stress proteins in L6 cells. GLUT4, IRE1‐alpha and TRAF2 proteins were analyzed in myotubes treated with 0,75mM of palmitate during 1 to 12 hours. The results showed that palmitate caused a significant reduction of the GLUT4 protein content in all treatment periods (control: 99.99±4.3, 2 hours: 66.37±7.2*, 4 hours: 65.03±8.0*, 6 hours: 67.16±8.8*, 12 hours: 58.88±5.5*), the analysis also showed an increase of IRE1‐alpha protein within 4 hours of incubation (control: 100.00±0.0, 4 hours: 184.16±49.45*) and an important increase of TRAF2 protein at the first hour of treatment (control: 100.00±0.0, 1 hour: 125.20±9.07*). Results were expressed in arbitrary units, n=4 to 6, *P<0.05. These data showed that palmitate impaired GLUT4 expression what is preceded by activation of IRE1alpha/TRAF2 pathway. This data reinforces the proposition that FFA‐induced ER stress is associated with IR probably by reducing GLUT4 content through mechanisms not fully identified.Support: Fapesp 2010/09984‐5
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