IPR-179, a gelatinase inhibitor, is able to mitigate epileptogenesis in the rat

Abstract

In the present study the lipid content under normal conditions of a freshwater native green microalga Selenastrum sp. GA66 was found to augment significantly when subjected to stress factors, viz. nitrate and phosphate starvation and NaCl supplementation. With the aim of maximizing it further for production of biodiesel, three optimization experiments were conducted of which the preeminent one comprising of 3.5 g L−1 NaCl, 0.06 g L−1 nitrate and incubation period of 4 days resulted in >3 fold boost in the lipid content alongside a significant reduction in the incubation period. In order to curb the loss of biomass and lipid yield due to stress condition, biphasic optimization strategy was implemented resulting in 2.6 fold higher lipid yield with 2.2 fold higher productivity. Fatty acid profile analyses of the biodiesel samples have presented a higher degree of saturation conversely with a lower amount of unsaturation under the biphasic optimized condition. Fuel properties of the biodiesel were tested to fall within the restrictions set by the International standards of biodiesel. As a way of generalising this strategy, six different freshwater microalgae were grown in this condition and each of them showed >2 fold higher lipid yields and productivities thereby, favouring its acceptance as a mass strategy for lipid induction. The optimized medium indicated 16 times reduction in nitrate requirement resulting in ∼5 times cost reduction in the optimized condition thereby, supporting it to be an economically feasible and environmentally sustainable approach for biodiesel production.