Abstract
BackgroundThe aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation.MethodsWistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to the treatment and euthanization time: C30 and C60 (right leg–without IR), IR30 and IR60 (left leg-with IR).ResultsCT analysis showed more radiodensity in C60 compared with other groups, and IR60 showed more radiodensity than IR30. In histomorphometric analysis, C30 showed lower bone matrix values compared with IR30 and C60. Lacunarity analyses showed more homogeneous bone channel distribution in C30 than IR30. ATR-FTIR showed decrease in ratio of mature and immature crosslinks in IR30 compared with C30. Crystallinity Index was decrease in IR60 compared with C60. The Amide III + Collagen/HA ratio was increased in C60 compared with C30; however this ratio decreased in IR60 compared with IR30. Biomechanical analysis showed lower values in IR groups in both time.ConclusionsIR damaged bone quality and decreased stiffness. Moreover, the results suggested that the deleterious effects of IR increased in the late time points.
Highlights
Radiotherapy has been proven to be successful treatment for local and regional neoplastic lesions, but it may adversely affect normal tissues [1]
computed tomography (CT) analyses showed that control 60 days (C60) (1090.8 ± 50.6) had an increased radiodensity value, compared with those of the other groups control 30 days (C30) (478.14 ± 31.27), IR30 (436.42 ± 43.22) and IR60 (877.9 ± 106) (p < 0.01)
IR60 showed increased radiodensity compared with IR30 (p < 0.01) (Fig. 1c)
Summary
Radiotherapy has been proven to be successful treatment for local and regional neoplastic lesions, but it may adversely affect normal tissues [1]. Studies have shown that IR applied in treatment of primary and secondary bone malignancy leads to hypocellularity, alterations of the Haversian systems and bone matrices [7, 8]. These changes result in deteriorated bone formation, with decreased osteoblast proliferation and differentiation [9], induction to cell-cycle arrest and direct cell death [1], damage of microvascular structures [10] and decreased collagen production [11]. The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation
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