Abstract
This paper describes a new approach for the determination of carbamazepine and lamotrigine in biological samples by ionic liquid dispersive liquid-phase microextraction prior to high-performance liquid chromatography with ultraviolet detection. The effects of different ionic liquids (ILs) on the extraction efficiency of carbamazepine and lamotrigine were investigated. The highest extraction efficiencies of carbamazepine and lamotrigine were obtained using 30 ?L of 1-me-thyl-3-octylimidazolium hexafluorophosphate [C8MIM][PF6]. Several factors affecting the microextraction efficiency, such as the type and volume of extracting solvent, type and volume of disperser solvent, salt concentration, and pH of the sample solution have been optimized. The calibration plots were linear in the range of 0.1-20 mg L-1 for carbamazepine and 0.3-40 mg L-1 for lamotrigine with detection limits of 0.04 mg L-1 for carbamazepine and 0.07 mg L-1 for lamotrig-ine in plasma samples. The results confirm the suitability of the presented method as a sensitive method for the analysis of the target analytes in urine and plasma samples.
Highlights
One of the most common serious neurological disorders is epilepsy.[1]
For the first time, dispersive liquid–liquid microextraction (DLLME) method using Ionic liquids (ILs) as extraction solvent combined with high-performance liquid chromatography has been developed for the simultaneous determination of carbamazepine and lamotrigine in biological samples
The applicability of ionic liquid DLLME combined with HPLC was considered for the simultaneous determination of carbamazepine and lamotrigine in biological samples
Summary
One of the most common serious neurological disorders is epilepsy.[1]. Anti-epileptic drugs (AEDs) are the main form of treatment for epilepsy. The structure of carbamazepine and lamotrigine partial and generalized toxic-clonic seizures. A sample preparation step is generally required to extract, isolate, and concentrate the analytes of interest. Liquid-liquid extraction (LLE),[16] solid-phase extraction (SPE),[17] and stir bar-sorptive extraction (SBSE)[18] have been developed for the determination of CBZ in biological fluids. These methods are time consuming and require substantial amounts of toxic organic solvents. For the first time, DLLME method using IL as extraction solvent combined with high-performance liquid chromatography has been developed for the simultaneous determination of carbamazepine and lamotrigine in biological samples. The proposed method was successfully applied to determine carbamazepine and lamotrigine in biological samples
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
