Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

Hassan Y Aboul-Enein,Xian Xiang Sun,Cheng Jun Sun

doi:10.3390/s21000424

Abstract

A plastic membrane electrode for the determination of methacycline hydrochloride was fabricated based on the use of methacycline-tetraphenylborate as the electroactive substance, and dioctylphtalate as the plasticizing agent. It was found that the potential responses of the electrode were influenced by pH of the tested solution when pH>3. Under the condition of pH 2.6, the linear response range, slope (25ºC) and detection limit obtained were 3.0×10-2~6.0×10-6M, 52.9mv per decade and 3.4×10-6M ,respectively. The response time of the electrodes was

Highlights

Tetracyclines are widely used as broad spectrum antibiotics for high activity against most gram-positive and gram-negative bacteria
The performances of the electrode along with the other related methacycline-selective electrodes which were fabricated by using the respective ion-pair compounds,methacyclinesilicotungstate (MC-SiT) and methacycline-phosphotungatate (MC-PT),as the corresponding electroactive substance in the membrane are investigated experimentally, and the proposed electrode was successfully applied for the determination of MC.Cl in the pharmaceutical tablet formulations
In the previous experimental investigations [19,20,21], it is obvious that both kind of plasticizer selected and the membrane composition used can influence the response performances of a PVC membrane ion-selective electrode, if other properties of the electrode, e.g. selectivity or the pH response, are omitted

Summary

Introduction

Tetracyclines are widely used as broad spectrum antibiotics for high activity against most gram-positive and gram-negative bacteria. For the determination of methacycline hydrochloride, high performance liquid chromatography [8] and antibiotics-microbial assay [9] were recommended in some pharmacopoeias. Few studies on the method for determination of methacycline(MC) in pharmaceutical analysis are reported, including high performance liquid chromatography [10], spectrophotometry [11], phosphorimetry [12] and thin-layer chromatography [13,14]. The performances of the electrode along with the other related methacycline-selective electrodes which were fabricated by using the respective ion-pair compounds,methacyclinesilicotungstate (MC-SiT) and methacycline-phosphotungatate (MC-PT),as the corresponding electroactive substance in the membrane are investigated experimentally, and the proposed electrode was successfully applied for the determination of MC.Cl in the pharmaceutical tablet formulations

Methods

Results

Conclusion