Abstract
An ultraperformance liquid chromatography tandem mass spectrometric(UPLC-MS/MS) method was established for the simultaneous determination of residues of 8 classes of 14 non-steroidal anti-inflammatory drugs(NSAIDs) in animal tissues.The samples were extracted with acetonitrile-ethyl acetate(1 ∶ 1,V/V),defatted with n-hexane,and then purified by MCX solid phase extraction(SPE) cartridge.Analysis was carried out on UPLC-ESI-MS/MS working with multiple reaction monitoring mode with polarity switching.Limits of detection were between 3.0 μg/kg and 10.0 μg/kg,and limits of quantification were between 10.0 μg/kg and 25.0 μg/kg.At spiked levels of 10.0-1000.0 μg/kg,in bovine and swine muscle the recoveries were 62.9%-108.4% and 63.4%-117.0%,respectively,and relative standard deviations(RSD) of the method were less than 10% and 9%,respectively.
Published Version
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