Abstract
a vacuum must occur before fragmentation. Recently, detection limits of low picograms in the full scan mode, and low femtograms in the selected-ion mode, were obtained using a coupled SFC-high resolution mass spectrometer system [9]. Sample introduction is the most difficult aspect of trace analysis in SFC. The sampling modes most widely used require splitting of a set volume delivered from a high pressure sample loop. This requires rather highly concentrated samples. Recently, solute focusing methods utilizing a retention gap, analogous to such methods used in capillary gas chromatography, have been applied in capillary SFC [10]. Proper manipulation of pressure and temperature is essential to achieving good chromatographic performance upon injection of large sample volumes. With I-AL injection volumes, sub-ppm concentrations can be detected using an FID, and much greater detection limits can be achieved using more selective detectors.
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