Abstract
Cytoskeletal components are established players in protein trafficking in epithelial cells. The bicarbonate‐secreting epithelium of salivary ducts is equipped with several acid‐base transporters whose cellular distribution is altered after systemic acid‐base disturbances. Here we investigated the role of actin and tubulin in V‐ATPase and electrogenic NBC1 distribution under physiological and pathological conditions in vitro. Therefore, extracellular acidosis or alkalosis was induced in the presence or absence of cytochalasin D or colchicine.Disruption of the actin network differentially influenced constitutive trafficking and adaptive redistribution after acid‐base disturbances. Following cytochalasin D treatment all transporters failed to reach the membrane. Trafficking of NBCe1‐B is impaired after disruption of the tubulin network as well, whereas localisation of NBCe1‐A was not affected by colchicine treatment during acid‐base disturbances.The results indicate differential regulation of tubulin‐mediated trafficking for NBCe1‐A and ‐B, reflecting their differences in the direction of ion transport and localisation in a polarized epithelium in vivo.This work was supported by the DFG.
Published Version
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