Abstract
Summary In vitro inactivation of Chlorella fusca NADH-nitrate reductase can be obtained by preincubation of the enzyme with reduced nicotinamide adenine dinucleotide. ADP cooperates with NADH in this inactivation. Anaerobic experiments reveal that oxygen is required in order to obtain inactivation of nitrate reductase by preincubation with NADH, alone or in the presence of ADP. Moreover, inactivation is prevented if superoxide dismutase and/or catalase are included in the preincubation mixture. The presence of hydrogen peroxide in the preincubation mixture increases the sensitivity of nitrate reductase to its inactivation by NADH. In vivo inactivation of Chlorella fusca NADH-nitrate reductase by addition of methylamine to the cells appears to depend on the intracellular catalase activity levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
