Abstract
Introduction: Cyclooxygenase-2 (COX-2) overexpression by a primary tumor correlates with poor prognosis in breast cancer, including early spread to bone. Interleukin-8 (IL-8) stimulates osteoclastogenesis and resorption of bone, and elevated IL-8 levels predict early metastatic spread of breast cancer. The purpose of this study was to test our hypothesis that tumors that overexpress COX-2 induce IL-8 production. Methods: We co-transfected MCF-10A (non-malignant breast epithelial) cells, as well as MDA-231 (highly-metastatic human breast cancer) cell lines with a pSG5- COX-2 vector and pEF1a-Luc-IRES-Neo vector (luciferase reporter).
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